Coding

Part:BBa_K4769205:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


yqxM-sipW-tasA operon coding sequence from B. subtilis 3610


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1531
    Illegal PstI site found at 1419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1531
    Illegal PstI site found at 1419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1531
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1531
    Illegal PstI site found at 1419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1531
    Illegal PstI site found at 1419
    Illegal NgoMIV site found at 841
    Illegal NgoMIV site found at 1142
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 448
    Illegal BsaI.rc site found at 1032


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: atgtttcgattgtttcacaatcagc (+ desired overhangs) RV: ttaatttttatcctcgctatgcgc (+ desired overhangs)


References